Coding

Part:BBa_K3787000

Designed by: Leung Gabriel, Tam Ching Yeung, Wong Chun Hei   Group: iGEM21_HK_GTC   (2021-07-23)


PETase(WT)-MHETase

This part is a chimeric protein used to depolymerize PET into its constituting monomers. It consists of two enzymes, PETase and MHETase. The PETase part degrades PET into bis(2-hydroxyethyl) terephthalic acid (BHET), mono(2-hydroxyethyl) terephthalic acid (MHET), and terephthalic acid (TPA), while the MHETase part degrades MHET into TPA and ethylene glycol (EG). The C-terminus of PETase is linked to the N-terminus of MHETase using a 12 amino acid serine-glycine linker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/composite_part
Parameters
protein